Analysis Note

ControlDuctal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues

Application

Research Sub CategoryTranscription Factors

Detect Twist1 (Twist-related protein 1) using this Anti-Twist1 (Twist-related protein 1) Antibody validated for use in IH(P) & IC.

Research CategoryStem Cell Research

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Twist1 in NIH/3T3, A431, and HeLa cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Twist1 is the basic helix-loop-helix transcription factor that regulates embryonic progenitor and transformed tumor cell proliferation, migration and differentiation and is also important in limb development. It has been shown that twist1 expression is high in patients suffering from rheumatic inflammatory disorders with chronically activated T helper lymphocytes and may be used as a biomarker for chronic t-cell activation. Mutations in twist1 are often associated with craniofacial and limb abnormalities in humans with Saethre-Chotzen syndrome. Twist1 dimer affinity can modify the DNA binding affinity. Twist1 has also been implicated as a promoter of tumor growth, invasion and anti-cancer-drug resistance.

Immunogen

KLH-conjugated linear peptide corresponding to human Twist1 near the N-terminus.

Epitope: Near N-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Immunohistochemistry in ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues.

Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Twist1 in ductal breast fibroadenoma and stratified squamous epithelium and cartilage of human trachea tissues.

Specificity

This antibody recognizes Twist1 near the N-terminus.

Other homologies: Rat (92% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

21 kDa calculated